Site-Directed Mutagenesis

Provide by Customer:

• Plasmid DNA containing the target gene fragment.
• Information of material provided and instruction for the service.

Procedure:

• Only 1-3 adjacent bases can be mutated at any one time.
• The mutation is checked by bi-directional DNA sequencing.
• The mutant plasmid DNA is delivered in the original vector.

Deliverables:

• 10µg mutant plasmid DNA and corresponding bacterial stock.
• Documentation of the project.
• Turnaround time is 4-6 weeks.