What we can do
DNA Purification
Services
- Antibody Purification Service
- Bacteria E.codiv Competent Cell Preparation
- Custom Peptide Synthesis
- Custom-Made Monoclonal Antibody
- Rabbit Polyclonal Antisera Against PEPTIDE
- Rabbit Polyclonal Antisera Against PROTEIN
- DNA Paternity Testing
- DNA Purification
- ELISA
- Gene Synthesis
- Comprehensive Health Monitoring For Rat & Mouse
- Infectious Disease PCR Testing For Rat & Mouse
- Mouse and Rat Serology Testing by MFIA
- Custom Animal Serum, Whole Blood and Plasma
- Preparation of Custom Buffers and Solutions
- Protein Profiling Service for Cytokines
- Disease-Specific Protein Profiling Service
- Protein Profiling Service For Transcriptional Factors
- GST-or His Taq Protein Purification Service
- RNA Gene Expression For Multiplex
- RNA Gene Expression For Single Plex
- Site-Directed Mutagenesis
- SNP and Genotyping
- Subcloning
WHAT WE CAN DO
Purification of plasmid DNA from bacterial culture
i-DNA offers services to purify plasmid DNA from bacterial culture.
TO DO THIS:
Turnaround is 2 days.
STEP
01
Customer provides the bacterial culture and information like plasmid name, antibodies marker and size.
STEP
02
i-DNA inoculates the bacteria and grows overnight.
STEP
03
i-DNA performs plasmid DNA purification and provides customer the purified plasmid DNA/ report.
i-DNA offer 2 production scales:
- < 10µg yield (mini-prep)
- ≥ 30µg yield (mini-prep)
i-DNA also offer the following additional services only to the plasmid DNA that is purified by i-DNA.
Restriction Digest
This consists of
- Incubating purified plasmid DNA with specified restriction endonuclease(s),
- Running the restriction digest product onto an agarose with specified DNA molecular weight ladder,
- Taking the gel image and (iv) writing the report.
DNA Sequencing
We can provide
- End-sequencing for checking the insert and orientation
- Full-sequencing for checking the integruty of the insert sequence
Consider outsourcing your plasmid DNA purification, please contact i-DNA for a no-obligation consultation. All information provided will be kept strictly confidential.