GST-or His Taq Protein Purification Service

Provide by Customer:

• Plasmid DNA containing the target gene fragment.
• Information of material provided and instruction for the service.

Procedure:

• Amplification of target gene fragment from the plasmid DNA provided.
• Cloning of amplified PCR product into a shuttle vector for DNA sequencing and restriction digest check.
• Subcloning of the correct PCR product into an appropriate E.coli expression vector for GST-or His-fusion protein, depending on customer’s preference.
• Screening for the correct and high fusion protein expression bacterial clones.
• Large scale (1-5L) bacterial culture and protein purification of His-tagged protein using Ni-NTA column or GST-tagged protein using glutathione column.

Deliverables:

• 10µg recombinant protein expression plasmid DNA and corresponding bacterial stock.
• 5mg of fusion protein with >80% purity based on SDS-PAGE gel analysis.
• Documentation of the project.
• Turnaround time is 4-5 weeks.